Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7
cell line
MCF7
cell type
ER+ breast cancer cell line
genotype
sgNR2F2-2
chip antibody
ER (Millipore, #06-935)

Sequenced DNA Library

library_name
GSM6717016
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching, chromatin was prepared using SimpleChIP Enzymatic Chromatin Kit from cell signaling. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
44877165
Reads aligned (%)
98.7
Duplicates removed (%)
25.9
Number of peaks
1034 (qval < 1E-05)

hg19

Number of total reads
44877165
Reads aligned (%)
97.9
Duplicates removed (%)
26.5
Number of peaks
523 (qval < 1E-05)

Base call quality data from DBCLS SRA